Coding

Part:BBa_M36915:Design

Designed by: Rohit Talreja, Kelly Nguyen, Jacqueline Tao   Group: Stanford BIOE44 - S11   (2012-12-06)


Codes for the benzylalcohol acetyltransferase (BEAT) enzyme


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 58
    Illegal NgoMIV site found at 1203
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence was extracted from a flower but optimized for a host E. Coli bacteria (e.g. deleted repeated sequences, optimized GC content).


Source

The cDNA and amino acid sequences were developed by Natalia Dudareva et. al Dudareva, Natalia, John C. D'Auria, Kyoung Hee Nam, Robert A. Raguso, and Eran Pichersky. "Acetyl-CoA:benzylalcohol Acetyltransferase - an Enzyme Involved in Floral Scent Production in Clarkia Breweri." The Plant Journal 14.3 (1998): 297-304.

References